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January 28, 2025
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This course is part of Researcher's Guide to Omic Data Specialization
Instructor: Candace Savonen, MS
Included with
Recommended experience
Intermediate level
Some experience with basic biology
Recommended experience
Intermediate level
Some experience with basic biology
You will be able to understand the goals and general workflows of DNA based methods and know tools and resources you should consider.
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This course is a follow up course to "Researcher's Guide to Fundamentals of Omic Data" which dives into further detail about DNA informatics methods!
This course is for individuals who: - Have taken Researcher's Guide to Fundamentals of Omic Data - Have DNA data and don’t know what to do with it. - Want a basic overview of their DNA focused data type. - Want to find resources for processing and interpreting DNA data What this course will cover: - Fundamentals of DNA methods. - Resources you may consider looking into for your own purposes. - Questions you should ask yourself and your colleagues about your goals and experimental design. What this course will NOT cover: - Code needed to process your data. - Details about every type of experimental design. - Everything you’d need to know to make you a computational biologist. In other words we still highly encourage you to consult your informatics and computational colleagues, especially those who may have done any handling of data you are trying to learn about.
In this module we discuss what this course covers and encourage you to make your own goals for what you'd like to learn. Then we start with some generalities about DNA focused methods.
3 videos3 readings2 assignments1 discussion prompt
In this module we cover the details of whole genome sequencing and whole exome sequencing. We compare and contrast the methods and discuss what kinds of questions these data types might answer.
2 readings2 assignments1 discussion prompt
In this section we dive into DNA methylation sequencing methods, also known more specifically as bisulfite sequencing.
2 readings2 assignments1 discussion prompt
In this section we cover the basics of chromatin evaluation methods. We contrast and compare chromatin sequencing methods.
1 video2 readings2 assignments1 discussion prompt
In this module, we discuss details of the different ways chromatin can be assessed. We go through the basics of ATAC-seq, and scRNA-seq.
2 videos3 readings2 assignments
In this section we cover the basics of ChIP-seq and CUT & Tag and CUT & RUN.
1 video4 readings2 assignments
In this section, In this module we discuss when microarrays should be used for assaying DNA instead of sequencing methods. and then we will wrap up this course with a final quiz to assess your absorption of the material. We also point you to our Tool Glossary that has a list of tools you may want to consider for your next steps of your DNA omics study.
3 readings1 assignment2 discussion prompts
Fred Hutchinson Cancer Center unites individualized care and advanced research to provide the latest cancer treatment options while accelerating discoveries that prevent, treat and cure cancer and infectious diseases worldwide. Based in Seattle, Fred Hutch is an independent, nonprofit organization and the only National Cancer Institute-designated cancer center in Washington. We have earned a global reputation for our track record of discoveries in cancer, infectious disease and basic research, including important advances in bone marrow transplantation, immunotherapy, HIV/AIDS prevention and COVID-19 vaccines. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services and has network affiliations with hospitals in four states. Fred Hutch also serves as UW Medicine’s cancer program.
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